BMB509: Bioanalytical instrumentation
Study Board of Science
Teaching language: Danish or English depending on the teacher
EKA: N200004112, N200004102
Assessment: Second examiner: None
Grading: Pass/Fail, 7-point grading scale
Offered in: Odense
Offered in: Autumn
Level: Bachelor
STADS ID (UVA): N200004101
ECTS value: 5
Date of Approval: 12-04-2023
Duration: 1 semester
Version: Approved - active
Comment
DISCONTINUED - last offered Autumn 2023
- Exam attempts are held January 2024
- Exam attempts are held March 2024. The student is registered for the exam attempt.
- Exam attempts will be held in January 2025. The student is registered for the exam attempt.
Entry requirements
Academic preconditions
Students taking the course are expected to:
- Have knowledge of basic mathematics and physics that include logarithms and exponential functions, linear algebra, first order differential equations, basic statistics and basic physical principles of electrodynamics, -statics and optics, as taught at gymnasium-level.
- Have knowledge of basic math, physics, chemistry and cell biology as taught in the first year of the bachelor program, as well as the content of the courses BMB532 and BMB533
- Be able to apply the math and physics taught in the first year of the program.
Course introduction
The aim of the course is to enable the student to understand and apply selected methods for investigating biomolecular structure and function. Techniques such as microscopy, mass spectrometry and chromatography will be demonstrated.
The course builds on the knowledge acquired in the courses listed under academic preconditions below, and gives an academic basis for studying the topics of the bachelor project but also of courses advanced biophotonics and mass spectrometry, that are part of the degree.
In relation to the competence profile of the degree it is the explicit focus of the course to:
- Give competence in understanding the application of selected biochemical techniques to determine biomolecular structure and function as well as spatial distribution of biomolecules and molecular complexes in cells.
- Give skills to apply these techniques to solve a biological problem.
- Introduce microscopy, mass spectrometry and chromatography methods used in modern biochemistry and molecular cell biology, including practical work and demonstrations.
Expected learning outcome
The learning objectives of the course are that the student demonstrates the ability to:
- Know the anatomy and function of the main components of chromatographic equipment.
- Know the anatomy and function of the main components of a mass spectrometer.
- Know the principles behind chromatographic separation of biomolecules.
- Know the principles behind mass spectrometric analysis of biomolecules incl. LC-MS).
- Apply chromatographic principles to optimize chromatographic separations.
- Perform an interpretation of mass spectra.
- Outline experiments for chromatographic separation and mass spectrometric analysis (incl. LC-MS) of biomolecules.
- Interpret data obtained by microscopy, mass spectrometry and chromatography (incl. LC-MS) of biomolecules.
- Explain principles of relevant light-matter interaction, as radiation, absorption, reflection, refraction, diffraction or emission from molecules (i.e. fluorescence).
- Describe properties and application of fluorescence probes being used in biochemical and cellular research.
- Know the anatomy and function of light microscopes including a description of its optical components.
Content
The following main topics are contained in the course:
- Introduction to microscope optics and computer-assisted image analysis.
- Detection and characterization of biomolecules by microscopy.
- Visualization and localization of biomolecules in cells by microscopy techniques.
- Introduction to mass spectrometry.
- Introduction to chromatography.
- Chromatographic separation of biomolecules based on their physical-chemical properties.
- Qualitative and quantitative chromatographic and mass spectrometric analysis of biomolecules.
Literature
Examination regulations
Prerequisites for participating in the exam a)
Timing
Autumn
Tests
Lab exercises
EKA
N200004112
Assessment
Second examiner: None
Grading
Pass/Fail
Identification
Full name and SDU username
Language
Normally, the same as teaching language
Examination aids
To be announced during the course
ECTS value
0
Additional information
The prerequisite examination is a prerequisite for participation in exam element a)
Exam element a)
Timing
January
Prerequisites
Type | Prerequisite name | Prerequisite course |
---|---|---|
Examination part | Prerequisites for participating in the exam a) | N200004101, BMB509: Bioanalytical instrumentation |
Tests
Oral exam
EKA
N200004102
Assessment
Second examiner: None
Grading
7-point grading scale
Identification
Student Identification Card
Language
Normally, the same as teaching language
Duration
20 minutes
Examination aids
To be announced during the course
ECTS value
5
Indicative number of lessons
Teaching Method
At the faculty of science, teaching is organized after the three-phase model ie. intro, training and study phase.
- Intro phase - 20 hours
- Training phase: 20 hours, including 8 hours tutorials and 12 hours laboratory
The lectures introduce the concepts and set the stage for the lab activities in the training phase.
Activities during the study phase:
- Read textbook, notes, compendia, articles and slides from the lectures
- Home assignments
- Preparation for laboratory exercises
Teacher responsible
Name | Department | |
---|---|---|
Thomas J.D. Jørgensen | tjdj@bmb.sdu.dk | Biomedicinsk Massespektrometri og systembiologi |
Additional teachers
Timetable
Administrative Unit
Team at Educational Law & Registration
Offered in
Recommended course of study
Transition rules
Transitional arrangements describe how a course replaces another course when changes are made to the course of study.
If a transitional arrangement has been made for a course, it will be stated in the list.
See transitional arrangements for all courses at the Faculty of Science.